Welcome, Guest. Please login or register.
January 24, 2018, 02:49:34 AM

Login with username, password and session length

  • Total Members: 32204
  • Latest: JUANILD
  • Total Posts: 725509
  • Total Topics: 59151
  • Online Today: 163
  • Online Ever: 1421
  • (August 13, 2016, 05:18:44 AM)
Users Online
Users: 2
Guests: 108
Total: 110


Welcome to the POZ Community Forums, a round-the-clock discussion area for people with HIV/AIDS, their friends/family/caregivers, and others concerned about HIV/AIDS.  Click on the links below to browse our various forums; scroll down for a glance at the most recent posts; or join in the conversation yourself by registering on the left side of this page.

Privacy Warning:  Please realize that these forums are open to all, and are fully searchable via Google and other search engines. If you are HIV positive and disclose this in our forums, then it is almost the same thing as telling the whole world (or at least the World Wide Web). If this concerns you, then do not use a username or avatar that are self-identifying in any way. We do not allow the deletion of anything you post in these forums, so think before you post.

  • The information shared in these forums, by moderators and members, is designed to complement, not replace, the relationship between an individual and his/her own physician.

  • All members of these forums are, by default, not considered to be licensed medical providers. If otherwise, users must clearly define themselves as such.

  • Forums members must behave at all times with respect and honesty. Posting guidelines, including time-out and banning policies, have been established by the moderators of these forums. Click here for “Am I Infected?” posting guidelines. Click here for posting guidelines pertaining to all other POZ community forums.

  • We ask all forums members to provide references for health/medical/scientific information they provide, when it is not a personal experience being discussed. Please provide hyperlinks with full URLs or full citations of published works not available via the Internet. Additionally, all forums members must post information which are true and correct to their knowledge.

  • Product advertisement—including links; banners; editorial content; and clinical trial, study or survey participation—is strictly prohibited by forums members unless permission has been secured from POZ.

To change forums navigation language settings, click here (members only), Register now

Para cambiar sus preferencias de los foros en español, haz clic aquí (sólo miembros), Regístrate ahora

Finished Reading This? You can collapse this or any other box on this page by clicking the symbol in each box.

Author Topic: Waking up dormant cells  (Read 2846 times)

0 Members and 1 Guest are viewing this topic.

Offline John2038

  • Member
  • Posts: 1,529
  • Happiness is a journey, not a destination.
    • HIV Research News (Twitter)
Waking up dormant cells
« on: March 14, 2013, 02:17:14 AM »
By using cancer drug, Vorinostat, for two weeks, Prof Lewin had been able to turn on sleeping HIV-infected cells so they could be detected

Offline Dr.Strangelove

  • Member
  • Posts: 215
Re: Waking up dormant cells
« Reply #1 on: March 14, 2013, 02:51:26 AM »
Lewin also gave a talk at CROI

It's good to see a step in the right direction to tackle the dormant cells. But it seems that HIV lies dormant in different types of cells, not all of them can be awaken by Vorinostat. So, this is only a piece of the puzzle. Like always.

Offline geobee

  • Member
  • Posts: 351
Re: Waking up dormant cells
« Reply #2 on: March 14, 2013, 02:32:12 PM »
Not to mention it's carcinogenic. 

A lot of work is being done to wake up latent cells.  Google "Bryologs" for example (and there's a thread here about that somewhere).  Smart people (Drs. Siliciano, Margolis, Deeks, etc. )  I'm confident they'll find something that works and hopefully isn't too toxic.

Offline elf

  • Member
  • Posts: 634
Re: Waking up dormant cells
« Reply #3 on: March 14, 2013, 08:40:28 PM »
Latent reservoirs cleaning ''substances'' need to be tested, and it can take 10 years or more.

So, why not use meds that are considered safe, and are already widely used in clinical settings, like

1) Disulfiram /Antabuse


    DSF reactivated latent HIV-1 expression in the U1 cell line, but not in the J89GFP or ACH2 cell lines. Interestingly, we found that DSF significantly reduced phosphatase and tensin homolog (PTEN) protein levels in U1 cells and in resting CD4 T cells from HIV-negative donors. Decreased PTEN resulted in increased phosphorylation of protein kinase B (Akt) and activation of the Akt signaling pathway. Consistent with these finding, pharmacological inhibitors of Akt and nuclear factor-kappaB (NF-κB) block the latent HIV-1-reactivating activity of DSF. Furthermore, we show that HIV-1 expression in the U1 cell line could be activated by a small molecule inhibitor of PTEN or by siRNA knockdown of PTEN expression. Neither the J89GFP nor ACH2 cells express PTEN, explaining the lack of DSF effect on HIV-1 expression in both these cell lines.
    CONCLUSION: DSF reactivates latent HIV-1 expression via the Akt signaling pathway through depletion of PTEN.


2) Miltefosine / Impavido

    Miltefosine targets HIV infected macrophages, which play a role in vivo as long-lived HIV-1 reservoirs. The HIV protein Tat activates pro-survival PI3K/Akt pathway in primary human macrophages. Miltefosine acts by inhibiting the PI3K/Akt pathway, thus removing the infected macrophages from circulation, without affecting healthy cells.
Getting used to my breakfasts with a pill of Complera.

Offline geobee

  • Member
  • Posts: 351
Re: Waking up dormant cells
« Reply #4 on: March 14, 2013, 08:47:11 PM »
Dr. Deeks at UCSF is testing disulfiram / antabuse.

Offline Ann

  • Administrator
  • Member
  • Posts: 28,138
  • It just is, OK?
    • Num is sum qui mentiar tibi?
Re: Waking up dormant cells
« Reply #5 on: March 15, 2013, 05:05:52 AM »

1) Disulfiram /Antabuse

Elf, have you, or has anyone you know, ever taken Antabuse? It's pretty nasty stuff. For many people, even ingesting small (tiny) amounts of alcohol (such as found in cough syrups, OTC cold remedies, as well as quite a few foods) can set off the adverse reaction and make a person tied to puking into their toilet for the next several hours. Not to mention the headache etc.

You'd have to be pretty dedicated to reading every single label of anything you ingest while taking it. It may be considered "safe" but every single person I know who has taken it wouldn't wish it on their worst enemy. (Well, ok, one or two would wish that, but not most.)

Ick. I think I'd rather have to keep my hiv than to take that stuff for the length of time that would likely be required in order to do what they only think it might do. Antabuse has fallen out of favour in alcohol rehab circles (with good reason) and it sounds like "someone" is just trying to find a new use for it. Kinda like they did with AZT.
Condoms are a girl's best friend

Condom and Lube Info  

"...health will finally be seen not as a blessing to be wished for, but as a human right to be fought for." Kofi Annan

Nymphomaniac: a woman as obsessed with sex as an average man. Mignon McLaughlin

HIV is certainly character-building. It's made me see all of the shallow things we cling to, like ego and vanity. Of course, I'd rather have a few more T-cells and a little less character. Randy Shilts

Offline Jeff G

  • Administrator
  • Member
  • Posts: 17,062
  • How am I doing Beren ?
Re: Waking up dormant cells
« Reply #6 on: March 15, 2013, 08:40:51 AM »
Antabuse was prescribed in the 80's because it would raise your cd4 counts ... so some docs prescribed it off label for this purpose . I didn't last two weeks on it before I ingested some alcohol in cough syrup that I wasn't aware of ... it was awful .
HIV 101 - Basics
HIV 101
You can read more about Transmission and Risks here:
HIV Transmission and Risks
You can read more about Testing here:
HIV Testing
You can read more about Treatment-as-Prevention (TasP) here:
You can read more about HIV prevention here:
HIV prevention
You can read more about PEP and PrEP here
PEP and PrEP


Terms of Membership for these forums

© 2017 Smart + Strong. All Rights Reserved.   terms of use and your privacy
Smart + Strong® is a registered trademark of CDM Publishing, LLC.