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Author Topic: DNA technology provides novel strategy for delivery of complex anti-HIV agent  (Read 2075 times)

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Offline Cosmicdancer

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DNA electroporation technology successfully used to direct expression of anti-HIV immunoadhesins and to modulate their function in vivo

Scientists have applied synthetic DNA technology to engineer a novel eCD4-Ig anti-HIV agent and to enhance its potency in vivo, providing a new simple strategy for constructing complex therapeutics for infectious agents as well as for diverse implications in therapeutic delivery.

Scientists at The Wistar Institute have applied their synthetic DNA technology to engineer a novel eCD4-Ig anti-HIV agent and to enhance its potency in vivo, providing a new simple strategy for constructing complex therapeutics for infectious agents as well as for diverse implications in therapeutic delivery. This critical development was published online in the journal EBio Medicine.

The development of a safe and effective HIV vaccine has proven critically challenging. Researchers are exploring passive immunization of laboratory-produced immunoadhesins as well as traditional gene therapy methods for delivery of these complex therapeutic molecules. Immunoadhesins are designed antibody-like molecules specifically engineered to efficiently neutralize diverse forms of HIV by binding with high affinity to the virus envelope. (continue reading at the link below)

"These complex therapeutics are difficult to deliver through traditional strategies and achieving full activity in vivo using DNA technology is also challenging," said lead researcher David B. Weiner, Ph.D., executive vice president, director of the Vaccine & Immunotherapy Center and W.W. Smith Charitable Trust Professor in Cancer Research at The Wistar Institute. "We demonstrated that a combination of plasmids can be designed to produce a novel protein as well as its modifying enzyme, allowing them to collocate with each other and create a highly functional immunoadhesin."

Electroporation of synthetic DNA (DNA/EP) consists of the application of small, controlled directional electric currents into the skin or muscle to facilitate optimal uptake of DNA molecules and local production of the DNA-encoded proteins. Using this technology, Weiner and colleagues were able to achieve robust and long-term in vivo expression. A single injection of the synthetic DNA formulation produced functional eCD4-Ig for several months in a mouse model.

https://www.sciencedaily.com/releases/2018/09/180904140537.htm
Summer, 2007 - &$#@?
November, 2007 - Tested poz, 300,000 vl, 560 cd4
Feb, 2008 - 57,000 vl, 520 cd4, started Atripla
2/2008 - 5/2015 - undetectable on Atripla
May, 2015 - UD, switched to Complera
September, 2015 - UD, 980 cd4, switched to Stribild (Complera interacted with acid reflux medication)
January, 2016 - Stribild, UD, 950 cd4
June, 2016 - UD, 929 cd4

 


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