how was the character of anti-hiv statement at test during period?

[blue22]

I could not find where to open this topic,so apologise if it is wrong place.
I request the hiv+ members to tell us how the statement of anti-hiv was during first 45 days.(classic elisa)

was it negative during first 20 days and suddenly it changed to positive or was it slowly changed to positive?
and how did you realise it?

many thanks

[Jim Allen]

Hi

Please do not post outside this thread again, the other sections of this forum are for people living with HIV not for you to ask them questions.

If you had an exposure test when due and move on with your life.

Here's what you need to know in order to avoid hiv infection:
Use condoms for anal or vaginal intercourse, correctly and consistently, every time, no exceptions.

Keep in mind that some sexual practices which may be described as ‘safe’ in terms of HIV transmission might still pose a risk for transmission of other STI's, so please do get fully tested regularly and at least yearly for all STI's including but not limited to HIV and test more frequently if unprotected intercourse occurs

Also note that it is possible to have an STI and show no signs or symptoms and the only way of knowing is by testing.

More information on HIV Basics, PEP, TaSP and Transmission can be found through the links in my signature to our POZ pages, this includes information on HIV Testing

Kind regards

Jim

Please Note.
As a member of the AM I Infected Forum you are required to only post in this one thread no matter how long between visits or the subject matter. You can find this thread by going to your profile and selecting show own post and it will take you here . It helps us to help you when you keep all your thoughts or questions in one thread and it helps other readers to follow the discussion. Any additional threads will be deleted.

[blue22]

hi jim;

the 41st day hiv rna pcr is negative ,now can I be sure that I was not infected?

[Jim Allen]

What was your exposure?

Also test with an approved antibodies test if you had an exposure, 6 weeks and 13 weeks post last possible exposure for a conclusive result

Here's what you need to know in order to avoid hiv infection:
Use condoms for anal or vaginal intercourse, correctly and consistently, every time, no exceptions.

Keep in mind that some sexual practices which may be described as ‘safe’ in terms of HIV transmission might still pose a risk for transmission of other STI's, so please do get fully tested regularly and at least yearly for all STI's including but not limited to HIV and test more frequently if unprotected intercourse occurs

Also note that it is possible to have an STI and show no signs or symptoms and the only way of knowing is by testing.

More information on HIV Basics, PEP, TaSP and Transmission can be found through the links in my signature to our POZ pages, this includes information on HIV Testing

Kind regards

Jim

Please Note.
As a member of the AM I Infected Forum you are required to only post in this one thread no matter how long between visits or the subject matter. You can find this thread by going to your profile and selecting show own post and it will take you here . It helps us to help you when you keep all your thoughts or questions in one thread and it helps other readers to follow the discussion. Any additional threads will be deleted.

[blue22]

hi jim ;

may I ask; are you a doctor for infectious diseases?
my exposure was oral sex (with a man) only. but there was wounds in my mouth.
I have tested today with approved antibodies

the results
**********************
Anti HIV 1+2 : 0.243
HBs Ag: 0.475
Anti HCV : 0.041
Anti Hbc IgG:2,16

**********************
I would tell a thing: one month before, I was delivering my pee pattern to laboratory but one woman crashed to my right arm,I had felt very little wettness on my arm (only like a point there) ,I then went to until gasılh,and washed  it with a detergent.

but some drops got into my eyes.

should I fear from this event (that means whether I might have been infected)




I do not know why but really I  have been feelling myself very very depressed and stressed.

and last months results are a bit different than now

last month (one month before)

Anti hiv: 0,09
Anti HBs : 0,18
vdrl -rpr : negative

summary:

my exposure was on 17th april
I have been tested with hiv RNA  8 ,15 and 41 days past exposure and all of these were negative

62 days after exposure : antihiv given above (0,09)
and todays results (91 days after/past exposure):given above. negative but from 0,09 to 0,241 in one month


there is a detail that I would point out : someone are telling about ELITE CONTROLLER

could you all of these and the event given above (might I be infected)?

stress stress stress

probbaly I have given around 22 tube bloods until now. and I feeling also boring than this but no extra way

there exist speculations between doctors' sayings.while  one of them says that I  was not infected another one says that I have to make test 6 months after exposure too

which of these is correct?

moreover ,I am using prescribed pschiayric drugs longer than 13 years. (now abilify maintena (400 mg),cedrina 50 mg xanax 0.5 mg. and I do not know whether these are affecting test results.

......

sorry for telling much thing. 

[Jim Allen]

my exposure was oral sex (with a man) only. but there was wounds in my mouth.

So you had no reason to stress or run out and test, regardless you did and the result is negative no surprise. Its time to put this behind you and to be honest if you can't you should be bringing this up with your therapist regarding coping with your thoughts instead of allowing yourself to act on fear.

Even if you had open bleeding gaping wounds in your head, saliva is a barrier and its hostile towards HIV braking down the receptors leaving HIV damaged and unable to infect, its such a near negligible concern we don't recommend testing over this. Simply move on and test whenever next due.

I would tell a thing: one month before, I was delivering my pee pattern to laboratory but one woman crashed to my right arm,I had felt very little wettness on my arm (only like a point there) ,I then went to until gasılh,and washed  it with a detergent.

but some drops got into my eyes.
should I fear from this event (that means whether I might have been infected)

Urine is not HIV infectious and on top of that I will explain that biologically, four conditions need to be present for transmission to occur: The virus must be present in an infectious body fluid from the HIV positive person, it must be present at sufficient levels to cause infection. There must be an effective route of transmission, and it must reach susceptible cells in another person.

You did not meet any of the 4 biological conditions needed and all 4 are needed.
You fear falls under what we call "spillage" contact with a source outside the human body and with a fluid that did not contain HIV to start with - You had no exposure

HiV is fragile and difficult to transmit so much so that well nobody has been infected by it outside the human body through contact and we know back to basics that HIV has an outer protein/receptors that are needed to remain infectious and they corrodes in contact with air, so it can no longer infect.

This poses a great problem to study it, and laboratory studies such as the ones at the CDC labs have to use unnaturally high and artificial laboratory-grown concentrations under precisely controlled and limited laboratory conditions to even study it, as it normally simply does not survive long in outside the body.  (Ask a lab how they do it, if you want to know more on how they artificially grow HIV as that part is beyond me)

Now the fact that in labs they can keep HIV viable outside the body has caused some people to misunderstand this to mean environmental risk is possible.
This is not the case as the labs use artificial conditions and concentrations of HIV many times greater than than anything ever found in patient specimens, the amounts of virus studied are simply not found in nature, and again no one has been infected with HIV this way.

You will not be making human history today with some pee that contained no HIV to start with that than was also exposed outside the human body.

my exposure was on 17th april
I have been tested with hiv RNA  8 ,15 and 41 days past exposure and all of these were negative

62 days after exposure : antihiv given above (0,09)
and todays results (91 days after/past exposure):given above. negative but from 0,09 to 0,241 in one month

Well RNA testing a bit pointless but the HIV antibodies test 3 months post exposure is conclusive, you had no exposure and no reason to test and you have a negative HIV result.

Congratulations you did not get HIV from this event Now relax move on with your life.

*1

there exist speculations between doctors' sayings.while  one of them says that I  was not infected another one says that I have to make test 6 months after exposure too

which of these is correct?

  ... Its been 3 months for about 20 to 25 years now. Not sure what you are on about.

there is a detail that I would point out : someone are telling about ELITE CONTROLLER

Someone is an idiot who is just winding you up with their own misunderstanding and outdated concepts. 

Elite controllers do test positive and it has nothing to do with a negative or positive HIV test . Elite controllers are nothing more than people with a low viral load, they still test positive on tests and their immune systems still produce the same antibodies.  A negative antibodies HIV test 3 months past any possible exposure is conclusive that you do not have HIV.

moreover ,I am using prescribed pschiayric drugs longer than 13 years. (now abilify maintena (400 mg),cedrina 50 mg xanax 0.5 mg. and I do not know whether these are affecting test results.

None of these will stop your immune system from producing antibodies and it will not change the HIV test result.


Finally we are just volunteers, people living with HIV who give their time free to provide information, some education and risk assessment as a service.

Your concerns and issues have nothing to do with HIV. I suggest you put this irrational fear behind you and if you can't than you continue to work with your therapist as looking back at all the tests, stress and everything you have put yourself through over nothing it is simply not productive or rational.

Jim

1.

Generation 1/2/3

Pilcher CD et al. Performance of Rapid Point-of-Care and Laboratory Tests for Acute and Established HIV Infection in San Francisco. PLOS ONE, 2013.

Branson BM State of the art for diagnosis of HIV infection. Clin Infect Dis 45:S221-225, 2007

Coombs RW Clinical laboratory diagnosis of HIV-1 and use of viral RNA to monitor infection. In Holmes KK (editor), Sexually Transmitted Diseases. New York: McGraw-Hill, 2008

Maldarelli F Diagnosis of Human Immunodeficiency Virus infection. In Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases (sixth edition). Philadelphia: Elsevier Churchill Livingstone, 2004

Parry JV et al. Towards error-free HIV diagnosis: guidelines on laboratory practice. Comm Dis Pub Health 6:334-350, 2003

3rd gen testing accuracy Perry KR et al. Improvement in the performance of HIV screening kits. Transfus Med 18:228-240, 2008

Generation 3 & 4
2015

https://www.ncbi.nlm.nih.gov/pubmed/25033879
(Blood drawn tests)
Abstract
Patients typically want accurate test results as soon as possible while clinicians prefer to wait until the probability of a false-negative is virtually nil. This review summarizes the median window periods for third-generation antibody and fourth-generation HIV tests and provides the probability of a false-negative result for various days post-exposure. Data were extracted from published seroconversion panels. The median (interquartile range) window period for third-generation tests was 22 days (19-25) and 18 days (16-24) for fourth-generation tests. The probability of a false-negative result is 0.01 at 80 days' post-exposure for third-generation tests and at 42 days for fourth-generation tests.

June 2016 write-up
http://i-base.info/guides/testing/appendix-1-different-types-of-hiv-test

Generation 4

https://www.cdc.gov/hiv/testing/clinical/index.html
2018
CDC recently published research findings that estimate the window period for 20 U.S. Food and Drug Administration (FDA)-approved HIV tests. The study showed that laboratory testing using antigen/antibody tests detects HIV infection sooner than other available tests that detect only antibodies. If a person gets a laboratory-based antigen/antibody test on blood plasma less than 45 days after a possible HIV exposure and the result is negative, follow-up testing can begin 45 days after the possible HIV exposure. For all other tests, CDC recommends testing again at least 90 days after exposure to be sure that a negative test result is accurate.

Bentsen C Performance evaluation of the Bio-Rad Laboratories GS HIV Combo Ag/Ab EIA, a 4th generation HIV assay for the simultaneous detection of HIV p24 antigen and antibodies to HIV-1 (groups M and O) and HIV-2 in human serum or plasma. Journal of Clinical Virology, S57-S61, 2011

Nick S Sensitivities of CE-Marked HIV, HCV, and HBsAg Assays. Journal of Medical Virology, S59-S64, 2007

Eshelman S Detection of Individuals With Acute HIV-1 Infection Using the ARCHITECT HIV Ag/Ab Combo Assay. Journal of Acquired Immune Deficiency Syndromes, 121-4, 2009

Speers D et al. Combination assay detecting both Human Immunodeficiency Virus (HIV) p24 antigen and anti-HIV antibodies opens a second diagnostic window. J Clin Microbiol 43:5397-5399, 2005

Ly TD et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods 122:185-94, 2004

http://www.bhiva.org/
Testing guidelines bhiva "British HIV association" : Although fourth generation tests shorten the time from exposure to seroconversion a repeat test at three months is still recommended to definitively exclude HIV infection.


2015 WHO http://apps.who.int/iris/bitstream/handle/10665/179870/9789241508926_eng.pdf;jsessionid=1F192FECF734A0DE7E2520864984AE63?sequence=1
In many settings post-test counselling messages recommend that all people who have a
non-reactive (HIV-negative) test result should return for retesting to rule out acute
infection that is too early for the test to detect (in other words, in the window period.
- 3 months) However, retesting is needed only for HIV-negative individuals who report recent or ongoing risk of exposure. For most people who test HIV-negative, additional retesting to rule out being in the window period is not necessary and may waste resources.

[Jim Allen]

Here is the summary version and overall take away for you.

You did not acquire HIV from the concerned incident and have tested conclusively  negative. There is nothing more to it than that, so relax, stop looking for issue you don't have and move on with you life.

Remember continue to use condoms for any intercourse and test out of standard routine at least yearly for easier to transmit STI's & HIV

Jim