Quantcast

Subscribe to:
POZ magazine
E-newsletters
Join POZ: Facebook MySpace Twitter Pinterest
Tumblr Google+ Flickr MySpace
POZ Personals
Sign In / Join
Username:
Password:
Welcome, Guest. Please login or register.
September 20, 2014, 02:54:23 AM

Login with username, password and session length


Members
  • Total Members: 23507
  • Latest: Zopper
Stats
  • Total Posts: 639524
  • Total Topics: 48545
  • Online Today: 179
  • Online Ever: 585
  • (January 07, 2014, 02:31:47 PM)
Users Online

Welcome


Welcome to the POZ/AIDSmeds Community Forums, a round-the-clock discussion area for people with HIV/AIDS, their friends/family/caregivers, and others concerned about HIV/AIDS.  Click on the links below to browse our various forums; scroll down for a glance at the most recent posts; or join in the conversation yourself by registering on the left side of this page.

Privacy Warning:  Please realize that these forums are open to all, and are fully searchable via Google and other search engines. If you are HIV positive and disclose this in our forums, then it is almost the same thing as telling the whole world (or at least the World Wide Web). If this concerns you, then do not use a username or avatar that are self-identifying in any way. We do not allow the deletion of anything you post in these forums, so think before you post.

  • The information shared in these forums, by moderators and members, is designed to complement, not replace, the relationship between an individual and his/her own physician.

  • All members of these forums are, by default, not considered to be licensed medical providers. If otherwise, users must clearly define themselves as such.

  • Forums members must behave at all times with respect and honesty. Posting guidelines, including time-out and banning policies, have been established by the moderators of these forums. Click here for “Am I Infected?” posting guidelines. Click here for posting guidelines pertaining to all other POZ/AIDSmeds community forums.

  • We ask all forums members to provide references for health/medical/scientific information they provide, when it is not a personal experience being discussed. Please provide hyperlinks with full URLs or full citations of published works not available via the Internet. Additionally, all forums members must post information which are true and correct to their knowledge.

  • Product advertisement—including links; banners; editorial content; and clinical trial, study or survey participation—is strictly prohibited by forums members unless permission has been secured from POZ.

To change forums navigation language settings, click here (members only), Register now

Para cambiar sus preferencias de los foros en español, haz clic aquí (sólo miembros), Regístrate ahora

Finished Reading This? You can collapse this or any other box on this page by clicking the symbol in each box.

Author Topic: Viral replication may not be primary cause of HIV-1 persistence in patients.  (Read 1023 times)

0 Members and 1 Guest are viewing this topic.

Offline Tadeys

  • Member
  • Posts: 159
(Medical Xpress)—A team of researchers with members from Europe and the U.S. has found that viral replication may not be the main reason that the HIV virus is able to persist in the cells of infected patients for many years. In their paper published in Proceedings of the National Academy of Sciences, the researchers outline a study they conducted with volunteer HIV infected patients and the results they found that indicated there is likely another cause for the long term persistence of HIV in human patients.

 
Over the years since the HIV virus made itself known in the form of AIDS, medical researchers have made dramatic gains in treating the disease. No longer is a positive test a death sentence. This has been made possible by the development of a cocktail of drugs known collectively as combinational antiretroviral therapy (cART). While it doesn't wipe the virus from the body, it does chase it into hiding. What has confounded researchers is the mechanism by which the virus hides in the body—quite often for several years, before suddenly one day revealing itself in the form of an outbreak. As part of this new effort, the research team believes they have taken a step forward in understanding how the HIV virus hides, and then, what causes it to become active again.
In the study, eight HIV positive patients who had been receiving cART for at least 12 years, volunteered to assist in the research effort. Each gave blood samples which were analyzed in a new way. In so doing the researchers found that each of the patients had a pool of infected CD4 cells—they are responsible for alerting T-cells when a foreign body is detected. The HIV virus had inserted its DNA into such cells in just such a way as to allow it to remain hidden in the body for long periods of time. It was only when some other foreign element entered the system that the CD4 cells were engaged. That in turn set off the HIV virus cells.
This, the researchers suggest, explains why a patient can go for years without any noticeable signs of HIV, then one day suddenly have a blow up. It's because some other virus or bacteria has made its way into the body causing the immune system to go on the attack. When it did so, it unwitting unleashed the HIV virus cells as well.
The researcher's findings suggest that the HIV virus doesn't need to replicate to remain active in the body for long periods of time as has been suspected.
 Explore further: Scientists find the invisibility cloak that shields HIV-1 from the immune system.


More information: The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time, Published online before print November 25, 2013, DOI: 10.1073/pnas.1308313110
Abstract
The source and dynamics of persistent HIV-1 during long-term combinational antiretroviral therapy (cART) are critical to understanding the barriers to curing HIV-1 infection. To address this issue, we isolated and genetically characterized HIV-1 DNA from naïve and memory T cells from peripheral blood and gut-associated lymphoid tissue (GALT) from eight patients after 4–12 y of suppressive cART. Our detailed analysis of these eight patients indicates that persistent HIV-1 in peripheral blood and GALT is found primarily in memory CD4+ T cells [CD45RO+/CD27(+/−)]. The HIV-1 infection frequency of CD4+ T cells from peripheral blood and GALT was higher in patients who initiated treatment during chronic compared with acute/early infection, indicating that early initiation of therapy results in lower HIV-1 reservoir size in blood and gut. Phylogenetic analysis revealed an HIV-1 genetic change between RNA sequences isolated before initiation of cART and intracellular HIV-1 sequences from the T-cell subsets after 4–12 y of suppressive cART in four of the eight patients. However, evolutionary rate analyses estimated no greater than three nucleotide substitutions per gene region analyzed during all of the 4–12 y of suppressive therapy. We also identified a clearly replication-incompetent viral sequence in multiple memory T cells in one patient, strongly supporting asynchronous cell replication of a cell containing integrated HIV-1 DNA as the source. This study indicates that persistence of a remarkably stable population of infected memory cells will be the primary barrier to a cure, and, with little evidence of viral replication, this population could be maintained by homeostatic cell proliferation or other processes.
Journal reference: Proceedings of the National Academy of Sciences 
view popular.
   
http://medicalxpress.com/news/2013-11-viral-replication-primary-hiv-persistence.html
       

Offline GoForIt

  • Member
  • Posts: 94
Yet another barrier as we continue to learn.
08/09/2013   Diagnosed WB positive
08/20/2013   CD4-506(28%)  VL-10,800
09/12/2013   CD4-391(28%)  VL-14,900
09/17/2013   Start ART (Truvada & Tivicay)
10/11/2013   CD4-377(26%)  VL-UD
12/20/2013   CD4-590(??%)  VL-UD
03/18/2014   CD4-660(29%)  VL-UD
07/22/2014   CD4-613(29%)  VL-UD
08/01/2014    Start TAF Clinical Trial

Offline Tadeys

  • Member
  • Posts: 159
" It's because some other virus or bacteria has made its way into the body causing the immune system to go on the attack. When it did so, it unwitting unleashed the HIV virus cells as well."

Another barrier? Don't think so, but don't know either. In a recent post on here (posted about a week ago?) some team in England learned that some oral bacteria can wake up the latent cells.

Think this news is somewhat positive...anybody have any thoughts?

Offline GoForIt

  • Member
  • Posts: 94
This study indicates that persistence of a remarkably stable population of infected memory cells will be the primary barrier to a cure, and, with little evidence of viral replication, this population could be maintained by homeostatic cell proliferation or other processes.

Journal reference: Proceedings of the National Academy of Sciences 
view popular.
   
http://medicalxpress.com/news/2013-11-viral-replication-primary-hiv-persistence.html
       

This line is the barrier.  Even if they stop it from replicating they believe it could maintain itself using other ways.
08/09/2013   Diagnosed WB positive
08/20/2013   CD4-506(28%)  VL-10,800
09/12/2013   CD4-391(28%)  VL-14,900
09/17/2013   Start ART (Truvada & Tivicay)
10/11/2013   CD4-377(26%)  VL-UD
12/20/2013   CD4-590(??%)  VL-UD
03/18/2014   CD4-660(29%)  VL-UD
07/22/2014   CD4-613(29%)  VL-UD
08/01/2014    Start TAF Clinical Trial

Offline Dr.Strangelove

  • Member
  • Posts: 208
Yes, but that not news. We already know that latent cells can persist for many years.

The question is if viral replication does take place or not. I remember there were some other studies that indicate it does take place.
Somehow I have a feeling it won't play a role as for finding The Cure™. We have to find a way to wake up or attack the latent cells, no matter if they do replicate or not...

 


Terms of Membership for these forums
 

© 2014 Smart + Strong. All Rights Reserved.   terms of use and your privacy
Smart + Strong® is a registered trademark of CDM Publishing, LLC.