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Author Topic: Western Blot  (Read 529 times)

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Offline wolfter

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Western Blot
« on: February 16, 2012, 06:56:40 AM »
I'm currently working on a project where as I'm finally taking an entire file cabinet full of medical paperwork and chronicling it by date.  I've purchased several large 3 ring binders and have different categories....lab, reports, diagnosis...etc

It's really strange reading through and rediscovering my own history.  Much of it forgotten.  One item that I found very odd was that I had a western blot test done in the mid 90's.  My first reaction...was wtf?  Who the hell ordered that a decade after my infection?

As I read through the papers it indicated the test was performed to verify the diagnosis of Lyme Disease.  I guess at the time I wasn't concerned about the testing methods as I had the obvious "bulls eye" rash on my thigh and already knew I had the infection.  I just never realized Western Blot was used for anything other than HIV.

Is there a point to any of this?  Probably not.  Just found it interesting. ;D

Have a great day y'all

Wolfie
productivity breeds content

Offline weasel

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Re: Western Blot
« Reply #1 on: February 17, 2012, 06:36:08 PM »


     Hi   Wolfie ,
                      Seems they use  Western Blot for   THOUSANDS  of

      different BUGS !
                                                                Weasel  :-*
" Live and let Live "

Online Ann

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  • It just is, OK?
    • Num is sum qui mentiar tibi?
Re: Western Blot
« Reply #2 on: February 18, 2012, 08:47:00 AM »
I don't know about "thousands". I only know about WB testing for hiv, BSE (mad cow disease), Lyme disease, hbv, herpes and FIV (feline immunodeficiency virus).

Did you guys know that there is also Northern Blot, Southern Blot and Eastern Blot? It's twoo! It's twoo!
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Offline weasel

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Re: Western Blot
« Reply #3 on: February 19, 2012, 10:57:59 AM »

I found this on the web . It appears to say  tens of thousands .
 Am I reading it right ?
Thanks , Weasel  :-*



The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in the given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.[2][3]
There are now many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins.[4] Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, biochemistry, immunogenetics and other molecular biology disciplines.
Other related techniques include using antibodies to detect proteins in tissues and cells by immunostaining and enzyme-linked immunosorbent assay (ELISA).
The method originated in the laboratory of George Stark at Stanford. The name Western blot was given to the technique by W. Neal Burnette and Sushant Bhat[5] and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed Northern blot.
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