Quantcast

Subscribe to:
POZ magazine
E-newsletters
Join POZ: Facebook MySpace Twitter Pinterest
Tumblr Google+ Flickr MySpace
POZ Personals
Sign In / Join
Username:
Password:
Welcome, Guest. Please login or register.
July 30, 2014, 03:09:13 AM

Login with username, password and session length


Members
Stats
  • Total Posts: 632347
  • Total Topics: 47886
  • Online Today: 227
  • Online Ever: 585
  • (January 07, 2014, 02:31:47 PM)
Users Online
Users: 2
Guests: 174
Total: 176

Welcome


Welcome to the POZ/AIDSmeds Community Forums, a round-the-clock discussion area for people with HIV/AIDS, their friends/family/caregivers, and others concerned about HIV/AIDS.  Click on the links below to browse our various forums; scroll down for a glance at the most recent posts; or join in the conversation yourself by registering on the left side of this page.

Privacy Warning:  Please realize that these forums are open to all, and are fully searchable via Google and other search engines. If you are HIV positive and disclose this in our forums, then it is almost the same thing as telling the whole world (or at least the World Wide Web). If this concerns you, then do not use a username or avatar that are self-identifying in any way. We do not allow the deletion of anything you post in these forums, so think before you post.

  • The information shared in these forums, by moderators and members, is designed to complement, not replace, the relationship between an individual and his/her own physician.

  • All members of these forums are, by default, not considered to be licensed medical providers. If otherwise, users must clearly define themselves as such.

  • Forums members must behave at all times with respect and honesty. Posting guidelines, including time-out and banning policies, have been established by the moderators of these forums. Click here for “Am I Infected?” posting guidelines. Click here for posting guidelines pertaining to all other POZ/AIDSmeds community forums.

  • We ask all forums members to provide references for health/medical/scientific information they provide, when it is not a personal experience being discussed. Please provide hyperlinks with full URLs or full citations of published works not available via the Internet. Additionally, all forums members must post information which are true and correct to their knowledge.

  • Product advertisement—including links; banners; editorial content; and clinical trial, study or survey participation—is strictly prohibited by forums members unless permission has been secured from POZ.

To change forums navigation language settings, click here (members only), Register now

Para cambiar sus preferencias de los foros en español, haz clic aquí (sólo miembros), Regístrate ahora

Finished Reading This? You can collapse this or any other box on this page by clicking the symbol in each box.

Author Topic: Gene Surgery at Temple U  (Read 1005 times)

0 Members and 1 Guest are viewing this topic.

Offline geobee

  • Member
  • Posts: 266
Gene Surgery at Temple U
« on: July 22, 2014, 12:40:15 AM »
They can target HIV inside the cell.  It works in the test tube.  Seems a long way from the clinic, but still, it's pretty cool!

"Dr. Khalili along with his colleague, Wenhui Hu, MD, PhD, the Associate Professor of Neuroscience at Temple, led the groundbreaking research, which was funded by grants from the National Institutes of Health. Khalili and his team created molecular tools to delete the HIV-1 proviral DNA. They used a combination of DNA-snipping enzyme called a nuclease and a targeting strand of RNA called a guide RNA(gRNA) to hunt down the viral genome and excise the HIV-1 DNA. The process allowed the cell’s gene repair machinery to take over, soldering the loose ends of the genome back together leading to virus-free cells."

Here's the link:

http://www.nbcphiladelphia.com/news/local/Groundbreaking-Research-Could-Lead-to-Cure-for-AIDS-267860541.html

Offline freewillie99

  • Member
  • Posts: 306
Re: Gene Surgery at Temple U
« Reply #1 on: July 22, 2014, 07:37:46 AM »
DNA-snipping enzyme called a nuclease

Sounds like a Zinc Finger nuclease.  Sangamo must be pleased.
Beware Romanians bearing strange gifts

Offline Almost2late

  • Member
  • Posts: 97
  • "My disease stops with me" - Jeff G
Re: Gene Surgery at Temple U
« Reply #2 on: July 22, 2014, 08:05:48 AM »
Found a link this morning with the same story and came running to share it but Geo beat me to it.. Hope it leads to a cure somewhere down the road..

http://6abc.com/health/temple-univ-team-makes-breakthrough-in-hiv-aids-research/206012/

 :)
Atripla, Bactrim, Azithromycin
Date         CD4's          VL
02/14     13  2.79%    228k+
03/14     52       7%       3k-
04/14     not done          2k-
05/14     184     9%       595
06/14     117     8%       235
End of June switched Meds
Tivicay, Truvada, Bactrim, Azithromycin

“HIV does not make people dangerous to know, so you can shake their hands and give them a hug:
Heaven knows they need it.”

Princess Diana

Offline Hoyland

  • Member
  • Posts: 71
Re: Gene Surgery at Temple U
« Reply #3 on: July 22, 2014, 09:59:55 AM »
This research is not a zinc finger project. The team is using much newer technology called CRISPR, which stands for “clustered   regularly interspaced short palindromic   repeats". This technology still has a long way to go before we see it in the clinic but, if it can be delivered to the right cells, it has the potential to cut out any piece of DNA in the genome. As HIV is a retrovirus it places its DNA into the host genome so any tool that can cut out this viral DNA has the potential to be a permanent cure for the disease.

Of course, cutting the wrong section of DNA could have catastrophic effects and this is why there will be a lot of water to flow under the bridge before we see this in humans.

ZFN has been around for about 16 years. CRISP has been around for about 2 years.

Offline aaware72

  • Member
  • Posts: 224
“It’s an important finding because for the first time in laboratory setting we show that the virus can be eradicated from human culture, cell culture, said Dr. Kamel Khalili, who led the research team. They developed molecular tools that can hunt down and delete HIV in cells. “Basically converting infected cells to un-infected cells and that is very important because the current therapy can not eliminate the virus from cells,” said Dr. Khalili."


http://philadelphia.cbslocal.com/2014/07/21/health-temple-university-researchers-successfully-eliminate-hiv-virus-in-human-cells/#.U83LJ_bOXDw.facebook
"Yes, knowledge is power. Self-knowledge brings mastery of one's body."

Offline zach

  • Member
  • Posts: 1,246
i just want video of hiv virus dying, withering and painful death, screams of turture... maybe directed by rob zombie

that would freaking make my day

Offline tednlou2

  • Member
  • Posts: 4,765
I just saw this on Facebook.  I kept wondering if the health reporter, Stephanie Stahl, is related to Leslie Stahl of "60 Minutes."  Of course, Leslie would have done a more in-depth look at the process.  But, good to see research is happening and researchers haven't given up, as illusive as it may be. 

Offline Miss Philicia

  • Member
  • Posts: 23,900
  • celebrity poster, faker & poser
There is already a thread on this: http://forums.poz.com/index.php?topic=54927.0

note: GO PHILLY!
"I’ve slept with enough men to know that I’m not gay"

Offline Jeff G

  • Administrator
  • Member
  • Posts: 10,831
  • How am I doing Beren ?
Re: Gene Surgery at Temple U
« Reply #8 on: July 22, 2014, 03:52:29 PM »
Thanks Miss P !

Offline zach

  • Member
  • Posts: 1,246
Re: Gene Surgery at Temple U
« Reply #9 on: July 22, 2014, 05:07:01 PM »
at some point i may well have to grudgingly admit that PHL must be a pretty cool place to be

Offline tryingtostay

  • Member
  • Posts: 143
i just want video of hiv virus dying, withering and painful death, screams of turture... maybe directed by rob zombie

that would freaking make my day

you and me both

lol
Labs:
March 2014: CD4 1730 @ 41%, VL 87 without meds
May 2014: CD4 1309 @ 42%, VL <20 without meds

Offline geobee

  • Member
  • Posts: 266
Re: Gene Surgery at Temple U
« Reply #11 on: July 23, 2014, 11:18:05 AM »
Here's a more optimistic article:

http://guardianlv.com/2014/07/hiv-and-aids-eradication-may-be-possible-with-new-treatment/

"Khalili admits further work must to be done in order to make the technique suitable for testing on human subjects, however he is confident that the eradication of HIV and AIDS may be possible with this new treatment."

I sure hope that this CRISPR thing recognizes *only* HIV.  Wouldn't want this thing inside you cutting up the wrong genes accidentally.

Offline tryingtostay

  • Member
  • Posts: 143
Re: Gene Surgery at Temple U
« Reply #12 on: July 23, 2014, 11:52:51 AM »
Here's a more optimistic article:

http://guardianlv.com/2014/07/hiv-and-aids-eradication-may-be-possible-with-new-treatment/

"Khalili admits further work must to be done in order to make the technique suitable for testing on human subjects, however he is confident that the eradication of HIV and AIDS may be possible with this new treatment."

I sure hope that this CRISPR thing recognizes *only* HIV.  Wouldn't want this thing inside you cutting up the wrong genes accidentally.

Yup.  I hope they would think of some kind of secondary fail safe method that would stop the thing from continuing.  Like something to mute or halt it if something goes wrong.  They should be developing that along side it's own creation.
Labs:
March 2014: CD4 1730 @ 41%, VL 87 without meds
May 2014: CD4 1309 @ 42%, VL <20 without meds

Offline Reggie

  • Member
  • Posts: 40
Re: Gene Surgery at Temple U
« Reply #13 on: July 23, 2014, 08:48:32 PM »
this is all good time being spent researching possibilities, hats off to those involved.

Offline Cosmicdancer

  • Member
  • Posts: 146
Re: Gene Surgery at Temple U
« Reply #14 on: July 24, 2014, 12:24:34 AM »
This gives a little more detail on how CRISPR works.

"To make sure CRISPR-Cas9 hits its HIV target, the team coupled it with an appropriately named escort: guide RNA (gRNA). The gRNA attaches to a very specific section of DNA, which is found only in HIV, ensuring that CRISPR-Cas9 can’t miss. The team screened for accidental, “off-target” editing and found virtually none. At least in the laboratory, the drug homes to and inactivates viral genes while leaving host DNA unchanged. Perhaps best of all, healthy cells containing the Cas9/gRNA complex were also immune to HIV infection in the experiment."

Revolutionary Biotech May Offer HIV Cure

By David Shultz

Earlier this month, an HIV infection reemerged in a 4-year-old Mississippi girl believed to have been cured of the virus. Researchers thought that by administering antiviral drugs quickly after she was born, they could destroy the virus before it could insert itself into her DNA.

For four years, it seemed the treatment had worked, but on July 10th officials announced that they had detected levels of the virus in her blood. Her story is representative of one of the tragic difficulties in curing HIV: The virus can hide in host DNA for long periods of time, evading drugs and the immune system alike. But a powerful new protein, known as CRISPR-Cas9, now has HIV’s clandestine genes in its sights.

HIV is often depicted as a spherical, free-floating virus coated with spiky protein receptors. This is accurate, but it only represents part of the virus’ life cycle. Like only imagining frogs as tadpoles, our depictions of HIV often fail to tell the whole story. Much of HIV’s life is spent inside our cells, lying dormant amidst our DNA. The reason we so rarely see an image of this latent version of the virus is because, at this point in its life, it’s made of nothing more than DNA itself. There is no viral shell, or membrane, or spiky protein receptors—just the genetic instructions for making them, sort of like a sleeper terrorist cell waiting to be activated.

It’s estimated that, even when no HIV particles are detectable in the body, around ten million cells carry genetic copies of the virus. As a result, the symptoms can be treated, but the infection can’t be cured.

That all stands to change if Kamel Khalili has his way. He and his team at Temple University have directed a protein called CRISPR-Cas9 to sniff out and remove latent HIV genes. The experiment took place in myeloid cells growing in culture -- an apt model, as these nervous system cells have proven to be a particularly good reservoir for HIV. When applied, Cas9 acts like a pair of molecular scissors that cut both ends of the HIV DNA, slicing it out of our chromosomes and preventing it from being used to make more viruses.

“It’s extremely specific, very efficient, and surprisingly, it does what you anticipate it should do,” said Khalili with a laugh. “We’ve actually converted the cell lines which carried the virus to be virus-free cells.”   

One of the hardest parts of removing viral genes is finding them. The HIV-1 genome is 9,181 base pairs; our own contains around 3 billion. Finding those viral genes is tantamount to finding a fire ant on a mile-long stretch of highway. The cost of missing the mark can be dangerous too. Accidentally altering important, healthy genes can result in cancer or other harmful side effects.

To make sure CRISPR-Cas9 hits its HIV target, the team coupled it with an appropriately named escort: guide RNA (gRNA). The gRNA attaches to a very specific section of DNA, which is found only in HIV, ensuring that CRISPR-Cas9 can’t miss. The team screened for accidental, “off-target” editing and found virtually none. At least in the laboratory, the drug homes to and inactivates viral genes while leaving host DNA unchanged. Perhaps best of all, healthy cells containing the Cas9/gRNA complex were also immune to HIV infection in the experiment.

The power of the CRISPR-Cas9 system has been awe-inspiring since its potential as a gene-editing tool was realized in 2012. Now, it seems these proteins may be capable of removing HIV infection at its root.

There are, of course, large differences between curing HIV in the lab, and doing it in the body. “It has the potential, but it’s a tall order to get [CRISPR-Cas9] into every cell of the human body,” says Khalili.  “We should be able to develop a strategy to effectively deliver this technology to infected individuals, and we would hope that it does the same thing.”

If his team is able to do that, then they may very well cure HIV.

Source: Hu W et al. "RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection." PNAS. Published online before print. doi: 10.1073/pnas.1405186111

http://www.realclearscience.com/articles/2014/07/23/revolutionary_biotech_may_offer_hiv_cure_108759.html

David Shultz is a freelance writer and an editorial intern at Nautilus Magazine. He tweets @dshultz14
Summer, 2007 - &$#@?
November, 2007 - Tested poz, 300,000 vl, 560 cd4
Feb, 2008 - 57,000 vl, 520 cd4, started Atripla
June, 2008 - undetectable, 612 cd4
January, 2009 - undetectable, 670 cd4
May, 2009 - undetectable, 593 cd4
Sept, 2009 - 83 vl, 763 cd4, 34%
Dec, 2009 - undetectable, 889 cd4, 32%
April, 2010 - undetectable, 860 cd4, 31%
October, 2010 - undetectable, 800 cd4, 38%
April, 2011 - undetectable, t-cell test not done
October, 2011 - undetectable
April, 2012 - undetectable, 850 cd4, 39%
November, 2012 - undetectable, 901 cd4, 41%
April, 2013 - undetectable, 846 cd4, 36%
October, 2013 - undetectable
May, 2014 - undetectable, 784 cd4, 48%

Offline tryingtostay

  • Member
  • Posts: 143
Re: Gene Surgery at Temple U
« Reply #15 on: July 24, 2014, 11:49:10 AM »
^ that's incredibly fascinating aside from the fact of my introspective look.
Labs:
March 2014: CD4 1730 @ 41%, VL 87 without meds
May 2014: CD4 1309 @ 42%, VL <20 without meds

Offline geobee

  • Member
  • Posts: 266
Re: Gene Surgery at Temple U
« Reply #16 on: July 24, 2014, 02:52:17 PM »
I agree.  Totally fascinating.  The idea of targeting a gene inside the body and changing it has huge implications for HIV and other genetic diseases.  It's mind-blowing.

Offline dico

  • Member
  • Posts: 72
Re: Gene Surgery at Temple U
« Reply #17 on: July 24, 2014, 04:36:38 PM »
But Sangamo just did that 5 years ago albeit with ZFN technology and not Cas9.

The issue is how to deliver them inside all latently infected cells... With the actual knowledge it is impossible to do that. All we can is taking some blood cells out of the body, treat them and reinject them to the body.

You can see that we aren't going far with that.

Offline geobee

  • Member
  • Posts: 266
Re: Gene Surgery at Temple U
« Reply #18 on: July 24, 2014, 06:21:00 PM »
This seems a little different than Sangamo in that their end goal is to modify the cells within the body instead of modifying your cells and then giving them back to you.   

 


Terms of Membership for these forums
 

© 2014 Smart + Strong. All Rights Reserved.   terms of use and your privacy
Smart + Strong® is a registered trademark of CDM Publishing, LLC.