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Author Topic: mutagenesis  (Read 2928 times)

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Offline powerpuff

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  • Posts: 138
mutagenesis
« on: June 13, 2007, 04:37:24 PM »
 :D a new drug outlook dealing with hiv genetic makeup cripples the virus to replicate. early stages don't know yet..anybody hear of it? or what stages... ???
mutagenesis sounds a bit like mutant x these days :D
intention to :cripple virus to replicate:// latent resevoirs or resting no idea?

Offline milker

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Re: mutagenesis
« Reply #1 on: June 13, 2007, 09:53:59 PM »
A link would be great !

Milker.
mid-dec: stupid ass
mid-jan: seroconversion
mid-feb: poz
mar 07: cd4 432 (35%) vl 54000
may 07: cd4 399 (28%) vl 27760
jul 07: cd4 403 (26%) vl 99241
oct 07: cd4 353 (24%) vl 29993
jan 08: cd4 332 (26%) vl 33308
mar 08: cd4 392 (23%) vl 75548
jun 08: cd4 325 (27%) vl 45880
oct 08: cd4 197 (20%) vl 154000 <== aids diagnosis
nov 2 08 start Atripla
nov 30 08: cd4 478 (23%) vl 1880 !!!!!!!!!!!!!!!!!!!!!!!!!!
feb 19 09: cd4 398 (24%) vl 430 getting there!
apr 23 09: cd4 604 (29%) vl 50 woohoo :D :D
jul 30 09: cd4 512 (29%) vl undetectable :D :D
may 27 10: cd4 655 (32%) vl undetectable :D :D

Now accepting applications from blowjob ninjas™

Offline motherinneed

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  • Posts: 76
Re: mutagenesis
« Reply #2 on: June 14, 2007, 11:26:04 AM »
3.Click here to read Links
    Lethal mutagenesis of HIV by mutagenic ribonucleoside analogs.
    Loeb LA, Mullins JI.

    Department of Pathology, University of Washington, Seattle 98195-7705, USA. laloeb@u.washington.edu

    The extraordinarily high mutation rate of HIV suggests that the viral population exists near the threshold for viral viability. An increase in mutation could mediate a lethal increase in the already high proportion of defective viruses. We initially tested this hypothesis by culturing HIV-infected cells in mutagenic deoxyribonucleoside analogs. In the presence of 5-hydroxydeoxycytidine, there was a loss of viral replication after sequential passages of HIV in human CEM cells (Loeb LA, et al.: Proc Natl Acad Sci USA 1999;96:14921497). We now propose to use mutagenic ribonucleosides to induce lethal mutagenesis. The incorporation of mutagenic analogs into the HIV genome by the host cell RNA polymerase, rather than by the viral reverse transcriptase, augurs well for the avoidance of resistant viruses.

    PMID: 10628810 [PubMed - indexed for MEDLINE]

Offline powerpuff

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  • Posts: 138
Re: mutagenesis
« Reply #3 on: June 14, 2007, 02:14:11 PM »
mutagenesis by dsbergermd@aol.com
chicago's nothstar clinic (773) 296-2400
i don't quite understand the process by which they cripple the virus by mutating ::)

puffy

Offline JPinLA

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  • Posts: 148
  • Cheers!
Re: mutagenesis
« Reply #4 on: June 14, 2007, 07:29:44 PM »
Hi again Puffy -

seems like they are giving providing a modified ribonucleosides to infected cells.  These are components necessary for RNA/DNA synthesis and replication of the viruses. 

The idea is that the modified nucleosides will be incporporated into replicating viral genome by the host cell's RNA polymerase during viral replication causing rapid rise in lethal mutations.  This polymerase is responsible for the incorporation of ribonuclesides into the new copies.

Since this incorporation would be mediated by the host cell (our) machinery (i.e. RNA polymerase) they contended that this would avoid viral resistance since it is outside the scope of the viral reverse transcriptase.

This study was done in 2000 and I have not yet done a search about the feasibility of such work in animals or humans since this was done in vitro.

My guess is that it has too many possibilites to cause back things for health cells (all type too..)

JP (who enjoys science)




11/06 - Diagnosed - VL/5784 & CD4 326
2/07 - VL/6000 & CD4 290 2/07
3//07 -Began Truvada/Viramune 
4/07 VL/undetectable and CD4 320 22%
7/07 VL/undetectable and CD4 286 22%
11/07 VL/undetectable and CD4 302 26%

Offline milker

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Re: mutagenesis
« Reply #5 on: June 14, 2007, 09:14:58 PM »
Ahhhhhhh fuck the ribosome in infected cells? Hmmm sounds dangerous, how does it select infected cells to start with, and there is a high risk of actually producing super-mutants, no?

Milker.
mid-dec: stupid ass
mid-jan: seroconversion
mid-feb: poz
mar 07: cd4 432 (35%) vl 54000
may 07: cd4 399 (28%) vl 27760
jul 07: cd4 403 (26%) vl 99241
oct 07: cd4 353 (24%) vl 29993
jan 08: cd4 332 (26%) vl 33308
mar 08: cd4 392 (23%) vl 75548
jun 08: cd4 325 (27%) vl 45880
oct 08: cd4 197 (20%) vl 154000 <== aids diagnosis
nov 2 08 start Atripla
nov 30 08: cd4 478 (23%) vl 1880 !!!!!!!!!!!!!!!!!!!!!!!!!!
feb 19 09: cd4 398 (24%) vl 430 getting there!
apr 23 09: cd4 604 (29%) vl 50 woohoo :D :D
jul 30 09: cd4 512 (29%) vl undetectable :D :D
may 27 10: cd4 655 (32%) vl undetectable :D :D

Now accepting applications from blowjob ninjas™

Offline JPinLA

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  • Posts: 148
  • Cheers!
Re: mutagenesis
« Reply #6 on: June 15, 2007, 10:43:17 AM »
I would think so.  Like I said thie research was done 7 years ago and I am not sure how much more has been done. I would think that this could have far reaching side effects as well as diffculty in delivery.

I don't know a lot about it, just what I read in the reference provided so I would have to do a little reading.  But, as I do puruse the literature a bit, I have not seen much recent news regarding this path for theraperutics.

JP
11/06 - Diagnosed - VL/5784 & CD4 326
2/07 - VL/6000 & CD4 290 2/07
3//07 -Began Truvada/Viramune 
4/07 VL/undetectable and CD4 320 22%
7/07 VL/undetectable and CD4 286 22%
11/07 VL/undetectable and CD4 302 26%

Offline powerpuff

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  • Posts: 138
Re: mutagenesis
« Reply #7 on: July 28, 2007, 03:36:07 PM »
they are doing trials now in chicago ;D
a pill form  that replicates itself to oblivion...

Offline J220

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  • Posts: 587
Re: mutagenesis
« Reply #8 on: July 28, 2007, 11:31:37 PM »
Intriguing idea I must say. To the virus: you like to mutate? Here, mutate yourself to oblivion.
"Hope is my philosophy
Just needs days in which to be
Love of Life means hope for me
Born on a New Day" - John David

Offline NYCguy

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  • Posts: 180
Re: mutagenesis
« Reply #9 on: July 30, 2007, 11:58:31 PM »
yes, I'm sure I read that there were trials of something using this philosophy going on, just not sure if it's the same thing.  There was a thread on here about it somewhere. Anyone find it?
11/9/06 = #$%^&!
sometime early Dec 2006:
CD4 530 20%/VL >250,000 (&*$$%!!)
started Reyataz300mg/Norvir/Truvada 12-27-06.
1/30/07 CD4 540 30%/VL <400
4/07 CD4 600+ 33%/VL <50
6/9/07 CD4 720 37%/VL <50
10/15/07 CD4 891 (!) %? VL <50
1/2010 CD4 599 (37%) VL<50 (drop due to acute HCV)
9/2010 - looks like HCV is gone for good! And I'm finally drinking again, thank GOD
2013 - considering a switch to Stribild. but I love my Kidneys (but I hate farting all the time!)...
June 2013 - switched to Stribild.  so far so good...

Offline milker

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Re: mutagenesis
« Reply #10 on: July 30, 2007, 11:59:16 PM »
Intriguing idea I must say. To the virus: you like to mutate? Here, mutate yourself to oblivion.
lol
mid-dec: stupid ass
mid-jan: seroconversion
mid-feb: poz
mar 07: cd4 432 (35%) vl 54000
may 07: cd4 399 (28%) vl 27760
jul 07: cd4 403 (26%) vl 99241
oct 07: cd4 353 (24%) vl 29993
jan 08: cd4 332 (26%) vl 33308
mar 08: cd4 392 (23%) vl 75548
jun 08: cd4 325 (27%) vl 45880
oct 08: cd4 197 (20%) vl 154000 <== aids diagnosis
nov 2 08 start Atripla
nov 30 08: cd4 478 (23%) vl 1880 !!!!!!!!!!!!!!!!!!!!!!!!!!
feb 19 09: cd4 398 (24%) vl 430 getting there!
apr 23 09: cd4 604 (29%) vl 50 woohoo :D :D
jul 30 09: cd4 512 (29%) vl undetectable :D :D
may 27 10: cd4 655 (32%) vl undetectable :D :D

Now accepting applications from blowjob ninjas™

 


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