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Author Topic: Robust CD4(+) and CD8(+) T cell responses to SIV using mRNA-transfected DC expre  (Read 1403 times)

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Offline hahaha

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  • Posts: 123
Robust CD4(+) and CD8(+) T cell responses to SIV using mRNA-transfected DC expressing autologous viral Ag
.Melhem NM, Liu XD, Boczkowski D, Gilboa E, Barratt-Boyes SM.
Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA, USA.

A potentially powerful strategy for therapeutic HIV vaccination is the use of DC transfected with mRNA encoding autologous viral Ag, as epitopes presented by transfected DC would exactly reflect those expressed by infected cells in the individual. Using human and rhesus macaque monocyte-derived DC, we show that nucleofection is a superior method for mRNA transfection, resulting in high-level protein expression and DC maturation. DC transfected with SIV gag isolated from an infected monkey stimulated robust Ag-specific recall T cell responses of similar magnitude to those induced by peptide-pulsed PBMC that were predominantly CD8(+) T cell mediated. Enhanced CD4(+) T cell responses were stimulated when Gag was redirected into the lysosomal pathway via the targeting signal derived from lysosome-associated membrane protein-1 (LAMP-1). Rhesus DC transfected with lysosome-targeted gag encoding an escape mutation in an immunodominant CTL epitope stimulated CD4(+) and CD8(+) T cell responses of almost equivalent magnitude directed towards undefined epitopes outside of the mutated region. Finally, gag-transfected DC from SIV-infected monkeys stimulated significant Ag-specific recall T cell responses in an entirely autologous system. These findings demonstrate that mRNA-transfected DC expressing SIV Ag derived from infected monkeys stimulate broad and relevant T cell responses, supporting this approach for therapeutic HIV vaccine development.
Aug 9, 2006 Get infected in Japan #$%^*
Oct 2006 CD4 239
Nov 2006 CD4 299 VL 60,000
Dec 1, Sustiva, Ziagan and 3TC
Jan 07, CD4 400

Offline J220

  • Member
  • Posts: 587
Interesting, when did this come out?
"Hope is my philosophy
Just needs days in which to be
Love of Life means hope for me
Born on a New Day" - John David

Offline hahaha

  • Member
  • Posts: 123
Published by J of Virology this month.  you may check on Pubmed.com, type keyword HIV therapeutic vaccine and search.
Aug 9, 2006 Get infected in Japan #$%^*
Oct 2006 CD4 239
Nov 2006 CD4 299 VL 60,000
Dec 1, Sustiva, Ziagan and 3TC
Jan 07, CD4 400

 


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