"Shape-shifting molecule tricks viruses into mutating themselves to death"

[tryingtostay]

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A newly developed spectroscopy method is helping to clarify the poorly understood molecular process by which an anti-HIV drug induces lethal mutations in the virus's genetic material. The findings from the University of Chicago and the Massachusetts Institute of Technology could bolster efforts to develop the next generation of anti-viral treatments.
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Viruses can mutate rapidly in order to adapt to environmental pressure. This feature also helps them become resistant to anti-viral drugs. But scientists have developed therapeutic anti-viral agents for HIV, Hepatitis C, and influenza using a strategy called lethal mutagenesis.

This strategy seeks to extinguish viruses by forcing their already high mutation rates above an intolerable threshold. If viruses experience too many mutations, they can't properly manage their genetic material.

"They can't replicate and so are quickly eliminated," said Andrei Tokmakoff, the Henry G. Gale Distinguished Service Professor in Chemistry at UChicago. "In order to make this work, you need a stealth mutagen. You need something sneaky, something that the virus isn't going to recognize as a problem."

Tokmakoff and his associates at UChicago and MIT reported new details of the stealthy workings of the anti-HIV agent KP1212 last month in the Proceedings of the National Academy of Sciences. Supporting data were collected with two-dimensional infrared spectroscopy, an advanced laser technique that combines ultrafast time resolution with high sensitivity to chemical structure.

Critical tool

"Two-dimensional infrared spectroscopy will be critical on the path ahead. It lets us look at the structures that exist in aqueous solution, which is the natural milieu of cells," said study co-author John Essigmann, MIT's William and Betsy Leitch Professor of Chemistry, Toxicology, and Biological Engineering. Essigmann is co-founder of a pharmaceutical company that is developing mutagenic inhibitors of HIV.

"We also have done nuclear magnetic resonance, which is very informative, but those studies were done in organic solvents that probably do not as accurately provide a view of what happens in cells as did the infrared studies done by the Tokmakoff group," Essigmann said.

Scientists design lethally mutagenic molecules such as KP1212 to resemble natural DNA bases, the adenine-thymine, cytosine-guanine base pairs. "These analogs can bind to the wrong base partners and therefore lead to genetic mutations," said the study's lead author, Sam Peng, who completed his doctorate at MIT in 2014.

KP1212 is a cytosine variation, which normally would pair with guanine during replication. But biochemical experiments and clinical trials have shown that KP1212 induces mutations by pairing with adenine. A leading proposal suggested that KP1212 derived its mutagenicity by shape shifting--converting into a different molecular structure by repositioning its hydrogen atoms on nitrogen and oxygen atoms.

Scientists call this shape-shifted structure a tautomer. James Watson, SB'47, and Francis Crick proposed this tautomer hypothesis in 1953 when they announced the discovery of DNA's double-helical structure. "The shuffled hydrogen positions in rare tautomers alter the hydrogen bonding patterns, resulting in incorrect base paring," said Peng, who will become a postdoctoral scientist at Stanford University later this year.

Rapid measurement

Most experimental tools would have difficulty distinguishing between the normal and shape-shifted structures because they interconvert very rapidly. With 2D infrared spectroscopy, the UChicago team was able to distinguish between the two structures. The team also was able to measure how rapid the shape shifting occurs under physiological conditions: in 20 billionths of a second.

The research team expected to find only two dominant tautomers, but their experiments showed that many more exist. In addition to taking on different forms as a neutral molecule, KP1212 also could accept an extra proton, giving it a positive charge at physiological levels of acidity (pH of approximately five and a half to seven) that made possible even more rearrangements and tautomer structures. "The number of possibilities exploded," Tokmakoff said.

The experiments also showed that both the protonated and non-protonated forms facilitated the viral mutation rate. Even in the absence of the protonated form, the virus still mutated, just at a lower rate.

"We found that under physiological pHs, KP1212 is significantly protonated and this protonated form induces even higher mutation rates, reaching approximately 50 percent," Peng said.

The finding that the molecule could become protonated both surprised and delighted Essigmann. The work taught his team how to create even more potent shape shifters--by decorating the KP1212 scaffold with groups of atoms and molecules that further raises their ability to capture protons.

"KP1212 is about 20 percent of the way toward being an ideal therapeutic mutagen. The hints given us by the spectroscopy guide us toward even better mutagenic molecules," Essigmann said.

Although Essigmann and Tokmakoff have known each other for years, they pursued seemingly far-removed research specialties until now. Tokmakoff's biological research involves proteins, not DNA. But together their research teams were able to fruitfully undertake one of the first 2D infrared spectroscopic studies of the therapeutic mechanism of an anti-viral drug.

[buginme2]

And? So?

[Cosmicdancer]

This drug, or an earlier version, KP-1461, has been kicking around for over a decade.  Then it was put on hold in 2008 because it showed no activity in a phase 2 human trial.

http://sfaf.org/hiv-info/hot-topics/beta/beta_2010_winspr_drugwatch.pdf

[buginme2]

How many of these articles are people going to post on here without saying a word about it.  Just a link and nothing else.  So what?  There's thousands of these on here and with each one it's like..look someones doing research.  Yay! 

If your going to post an article I would rather hear your opinion about it.  Say something!  Anything, even if you call it a bunch of bullshit (which most are really, including this one. I mean so what who cares).

Yeah that kp-whatever has been around for at least a decade and it went no where.  I suspect the same thing with this little nugget of hiv. 

[zach]

jennifer lawrence as mystique... now there is some shape shifting i'm interested in 

[Jeff G]

How many of these articles are people going to post on here without saying a word about it.  Just a link and nothing else.  So what?  There's thousands of these on here and with each one it's like..look someones doing research.  Yay! 

If your going to post an article I would rather hear your opinion about it.  Say something!  Anything, even if you call it a bunch of bullshit (which most are really, including this one. I mean so what who cares).

Yeah that kp-whatever has been around for at least a decade and it went no where.  I suspect the same thing with this little nugget of hiv. 

Im nobody and I support this message .

[geobee]

OK, I'll bite.

Seems like Koronis Pharma was never really happening as a company.  Their non-website is:

http://www.koronispharma.com/

They used to have a decent website with a video talking about KP1461 (I think) and accelerating viral mutation.  The idea being that you could make it less replication-competent. 

I've always been a little leery about encouraging mutation.  I mean, yikes.  What if you encouraged mutation, didn't do a good job at inhibiting replication, and now you had a resistant virus?  Always seemed like a dicey proposition to me.

[tryingtostay]

This tech is a long way off and probably won't be the answer but the idea of killing the virus with it's own mutation sounded pretty evil which in this case i would fully support.  In a way it's a little like how we get sick from the virus.  Very novel idea but it was entertaining reading this and worth a read. 

[tednlou2]

How many of these articles are people going to post on here without saying a word about it.  Just a link and nothing else.  So what?  There's thousands of these on here and with each one it's like..look someones doing research.  Yay! 

If your going to post an article I would rather hear your opinion about it.  Say something!  Anything, even if you call it a bunch of bullshit (which most are really, including this one. I mean so what who cares).

Yeah that kp-whatever has been around for at least a decade and it went no where.  I suspect the same thing with this little nugget of hiv.

There are studies that come out, which I don't understand enough to comment.  If I share one without comment, it is for information/archive purposes and/or to see what more knowledgeable members may say about it.